NGS-based donor chimerism monitoring after HSCT. These reports are built from the public SRP434573 mixtures (a public-data demonstration, not patient data). The titrated minor contributor (F2) is assigned the host role, so the monitored quantity is the host fraction.
The headline example: the 1% titration 1_99_F2-M1. The
estimate recovers the 1% host fraction cleanly.
Near the panel's contamination floor: the 0.5% titration
1_199_F2-M1. The MLE reads slightly low because of a
donor-homozygous contamination background in this public dataset; the
host-presence test still detects the residual host signal.
The whole F2-into-M1 titration ladder fed to timeline mode to show the trend chart. This is a titration series, not serial timepoints from one patient.